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Title
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Complex of lac repressor headpiece with a 14 base-pair lac operator fragment studied by two-dimensional nuclear magnetic resonance.
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Authors
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R.Boelens,
R.M.Scheek,
J.H.van Boom,
R.Kaptein.
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Ref.
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J Mol Biol, 1987,
193,
213-216.
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PubMed id
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Abstract
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Two-dimensional nuclear Overhauser enhancement spectra are presented of the
complex of lac repressor headpiece with a 14 base-pair lac operator fragment.
Analysis of nuclear Overhauser enhancements observed between protein and DNA
shows that the second helix of the headpiece ("the recognition helix") binds in
the major groove of DNA as has been suggested, but that the orientation of this
helix is approximately 180 degrees different from the proposed models.
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