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Title
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Complete sequence-specific 1H nuclear magnetic resonance assignments for mouse epidermal growth factor.
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Authors
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D.Kohda,
F.Inagaki.
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Ref.
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J Biochem (tokyo), 1988,
103,
554-571.
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PubMed id
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Abstract
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The 1H NMR spectrum of the mouse epidermal growth factor (53 residues) was
analyzed with the use of two-dimensional NMR techniques. All the observable 296
proton resonances were completely assigned in a sequential manner. For the spin
system identification, two-dimensional homonuclear Hartmann-Hahn spectrum was
useful, especially for arginine and proline residues. The easy spin system
identification of these long-side-chain-bearing amino acid residues greatly
facilitated the sequence-specific resonance assignment of the epidermal growth
factor.
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