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Title
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A comparative 1H NMR study of mouse alpha(1-53) and beta(2-53) epidermal growth factors.
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Authors
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D.Kohda,
C.Kodama,
R.Kase,
H.Nomoto,
K.Hayashi,
F.Inagaki.
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Ref.
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Biochem Int, 1988,
16,
647-654.
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PubMed id
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Abstract
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The three-dimensional structure of the mouse epidermal growth factor (EGF) in
solution was studied by comparison of the 1H NMR spectra of alpha EGF (1-53) and
beta EGF (2-53, des-asparaginyl 1 form). Using pH dependence of chemical shifts
and a two-dimensional difference spectrum, the effect of the N-terminal deletion
was investigated based on the complete assignment of the proton resonances. The
affected residues were all found to be located exactly in the triple-stranded,
beta-sheet core in the N-terminal domain of the EGF molecule.
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