 |
|
Title
|
|
The interaction of lac repressor headpiece with its operator: an NMR view.
|
|
|
Authors
|
|
R.Boelens,
R.M.Lamerichs,
J.A.Rullmann,
J.H.van Boom,
R.Kaptein.
|
|
|
Ref.
|
|
Protein Seq Data Anal, 1988,
1,
487-498.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Analysis of nuclear Overhauser enhancements in two-dimensional NMR spectra of
the complex of lac repressor headpiece with a 14 base pair lac operator fragment
shows that the second helix of the headpiece binds in the major groove of DNA,
as has been suggested. The orientation of this helix is approximately 180
degrees different from the proposed models and from that found in the X-ray
structure of the 434 repressor-operator complex. The model of the lac
headpiece-operator complex provides a good explanation for a large amount of
biochemical, genetic and physical data.
|
|
|
|