 |
|
Title
|
|
Systematic application of high-resolution, phase-sensitive two-dimensional 1H-NMR techniques for the identification of the amino-acid-proton spin systems in proteins. Rabbit metallothionein-2.
|
|
|
Authors
|
|
D.Neuhaus,
G.Wagner,
M.Vasák,
J.H.Kägi,
K.Wüthrich.
|
|
|
Ref.
|
|
Eur J Biochem, 1985,
151,
257-273.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Novel strategies for elucidation and classification of amino acid 1H-NMR spin
systems in proteins were developed exploiting recently introduced
two-dimensional NMR techniques such as phase-sensitive double-quantum-filtered
correlated spectroscopy, relayed coherence transfer spectroscopy, double quantum
spectroscopy and nuclear Overhauser spectroscopy. Due to the improved resolution
in phase-sensitive spectra, the fine structure of cross peaks could be exploited
as a powerful source of information for establishing 1H-1H connectivities.
Principles for the interpretation of multiplet structures of absorption mode
cross peaks are discussed. With these methods the 1H spin systems of rabbit
liver metallothionein-2 were elucidated and classified according to amino acid
types. Despite the intrinsically difficult situation arising from the unusual
amino acid composition of this protein, a more complete characterization of the
1H spin systems prior to the step of sequential resonance assignments was
achieved with the presently introduced methodology than was possible in earlier
studies of proteins of similar size.
|
|
|
|