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The Gram-negative bacterium Erwinia amylovora is a destructive pathogen of
plants belonging to the Rosaceae family. Amongst its pathogenicity factors, E.
amylovora produces the exopolysaccharide amylovoran, which contributes to the
occlusion of plant vessels, causing wilting of shoots and eventually resulting
in plant death. Amylovoran biosynthesis requires the presence of 12 genes (from
amsA to amsL) clustered in the ams region of the E. amylovora genome. They
mostly encode glycosyl transferases (AmsG, AmsB, AmsD, AmsE, AmsJ and AmsK),
proteins involved in amylovoran translocation and assembly (AmsH, AmsL and
AmsC), and also a tyrosine kinase (AmsA) and a tyrosine phosphatase (AmsI),
which are both involved in the regulation of amylovoran biosynthesis. The
low-molecular-weight protein tyrosine phosphatase AmsI was overexpressed as a
His6-tagged protein in Escherichia coli, purified and crystallized. X-ray
diffraction data were collected to a maximum resolution of 1.57 Å in space
group P3121.
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