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Title
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Two-dimensional 1H-NMR study of synthetic peptides containing the main immunogenic region of the Torpedo acetylcholine receptor.
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Authors
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M.T.Cung,
M.Marraud,
I.Hadjidakis,
E.Bairaktari,
C.Sakarellos,
A.Kokla,
S.Tzartos.
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Ref.
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Biopolymers, 1989,
28,
465-478.
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PubMed id
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Abstract
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A comparative 1H-NMR spectral study of a synthetic decapeptide containing the
main immunogenic region of the Torpedo acetylcholine receptor (AChR; WNPADYGGIK,
representing the alpha 67-76 fragment of Torpedo AChR) with four analogous
peptides (WNP3-D5YGGIK, WNPAA5YGGIK, WNPADYGGA9K, and WNPD4DYGGV9K) has been
carried out in dimethyl sulfoxide. One- and two-dimensional nmr experiments
[correlated spectroscopy (COSY), relayed COSY, and phase-sensitive nuclear
Overhauser enhancement spectroscopy (NOESY)] were performed to obtain complete
assignments of the proton resonances. The presence of strong and multiple short-
and long-range NOEs, and especially a strong long-range NOE between the two
Asn2-C alpha H and Gly7-C alpha H protons, argues in favor of a rigid folded
structure in all five cases. Temperature dependence measurements indicate the
existence of three intramolecular interactions involving the Asp3, Gly8, and
Lys10 amide protons.
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