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Title
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Crystallization of the Fab from a human monoclonal antibody against gp 41 of human immunodeficiency virus type I.
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Authors
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E.Casale,
E.Wenisch,
X.M.He,
P.G.Righetti,
R.S.Snyder,
A.Jungbauer,
C.Tauer,
F.Ruker,
D.C.Carter.
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Ref.
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J Mol Biol, 1990,
216,
511-512.
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PubMed id
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Abstract
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A monoclonal IgG antibody directed against gp 41 from the human immunodeficiency
virus (HIV-1) has been crystallized in both intact and Fab forms. Crystals of
the intact antibody grow as tetragonal-like prisms too small for conventional
X-ray analysis. However, the Fab portion of the antibody produces suitable
plate-like crystals which belong to the space group P2(1)2(1)2(1) with unit cell
constants of a = 66.5 A, b = 74.3 A and c = 105.3 A. There is one molecule of
Fab in the asymmetric unit. The Fab crystals show diffraction to d-spacings less
than 3.0 A.
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