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Title
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Crystallization of recombinant rat cathepsin B.
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Authors
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X.Lee,
F.R.Ahmed,
T.Hirama,
C.P.Huber,
D.R.Rose,
R.To,
S.Hasnain,
A.Tam,
J.S.Mort.
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Ref.
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J Biol Chem, 1990,
265,
5950-5951.
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PubMed id
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Abstract
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A glycosylation-minus mutant of rat cathepsin B expressed in yeast has been
purified and crystallized. X-ray diffraction data have been collected and
molecular replacement for solving the structure is in progress. The space group
for the recombinant rat cathepsin B was determined to be P2(1) with unit cell
dimensions alpha = 62.2 A, b = 90.19 A, c = 47.07 A, and beta = 97.43 degrees. A
unit cell contains 4 molecules and 2 molecules per asymmetric unit.
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