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Title
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Crystallization and preliminary x-ray diffraction studies of the Fab fragment of a neutralizing monoclonal antibody directed against human rhinovirus serotype 2.
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Authors
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J.Tormo,
I.Fita,
O.Kanzler,
D.Blaas.
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Ref.
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J Biol Chem, 1990,
265,
16799-16800.
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PubMed id
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Abstract
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We report on the preparation, crystallization, and preliminary x-ray diffraction
analysis of the Fab fragment of the monoclonal antibody 8F5 that neutralizes
infectivity of human rhinovirus serotype 2 (HRV2). Fab fragments prepared from
this antibody by papain digestion were purified to isoelectric homogeneity by
ion exchange chromatography and chromatofocusing. Crystals were obtained by the
hanging drop vapor diffusion method using ammonium sulfate as precipitant. The
crystals belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell
dimensions a = 59.9 A, b = 86.3 A, c = 128.2 A and diffract to at least 2.8-A
resolution. The cell volume suggests the presence of one molecule per asymmetric
unit, and the solvent content is estimated to be 61%.
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