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Title
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Crystallization and characterization of the high molecular weight form of nerve growth factor (7 S NGF).
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Authors
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N.Q.McDonald,
T.L.Blundell.
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Ref.
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J Mol Biol, 1991,
219,
595-601.
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PubMed id
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Abstract
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A high molecular weight form of nerve growth factor (7 S NGF) has been
crystallized in two crystal forms from polyethylene glycol 4000 by the vapour
diffusion technique. The orthorhombic form A belongs to the space group
P2(1)2(1)2(1) and has cell dimensions of a = 95.6, b = 96.5 and c = 147.0 A.
With synchrotron X-ray radiation, these crystals diffract to 2.8 A resolution.
They contain an intact 7 S NGF complex in the asymmetric unit. The tetragonal
form B, which grows at similar conditions to the A form, belongs to the space
group P4(1)2(1)2 (or P4(3)2(1)2) with unit cell dimensions of a = 97.4, b = 97.4
and c = 308.3 A. These crystals diffract to 3.6 A resolution and contain one 7 S
complex per asymmetric unit. Native X-ray data have been collected to 3.3 A for
the A form and to 5.0 A for the B form, both using synchrotron radiation.
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