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Title
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Cloning, expression, crystallization and preliminary X-ray analysis of the first two Ig domains from human roundabout 1 (Robo1).
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Authors
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C.Morlot,
W.Hemrika,
R.A.Romijn,
P.Gros,
S.Cusack,
A.A.McCarthy.
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Ref.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007,
63,
689-691.
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PubMed id
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Abstract
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Activation of Roundabout 1 (Robo1) by Slit proteins results in axon repulsion
from the midline. Robo1 is a large transmembrane receptor expressed on the axon
growth cone and the minimal Robo1-binding region required for Slit activation
has been mapped to the N-terminal Ig1-2 domains. The cDNA encoding the first two
Ig domains of Robo1 has been cloned and the protein has been expressed in HEK293
EBNA-1 mammalian cells. Here, the purification and crystallization conditions of
this Robo1 construct are reported. The crystals are orthorhombic, space group
P2(1)2(1)2, with unit-cell parameters a = 38.8, b = 69.4, c = 103.3 A and one
molecule in the asymmetric unit. X-ray diffraction data have been collected to
2.8 A resolution on beamline ID29 at the ESRF.
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