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Title
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Crystallization of hepatocyte nuclear factor 1beta in complex with DNA.
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Authors
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P.Lu,
Y.Li,
A.Gorman,
Y.I.Chi.
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Ref.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 2006,
62,
525-529.
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PubMed id
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Abstract
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Hepatocyte nuclear factor 1beta (HNF1beta) is a member of the POU
transcription-factor family and binds the target DNA as a dimer with nanomolar
affinity. The HNF1beta-DNA complex has been prepared and crystallized by
hanging-drop vapor diffusion in 6%(v/v) PEG 300, 5%(w/v) PEG 8000, 8%(v/v)
glycerol and 0.1 M Tris pH 8.0. The crystals diffracted to 3.2 A (93.9%
completeness) using a synchrotron-radiation source under cryogenic (100 K)
conditions and belong to space group R3, with unit-cell parameters a = b =
172.69, c = 72.43 A. A molecular-replacement solution has been obtained and
structure refinement is in progress. This structure will shed light on the
molecular mechanism of promoter recognition by HNF1beta and the molecular basis
of the disease-causing mutations found in it.
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