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Title
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Human ITCH is a coregulator of the hematopoietic transcription factor NF-E2.
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Authors
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X.Chen,
S.Wen,
M.N.Fukuda,
N.R.Gavva,
D.Hsu,
T.O.Akama,
T.Yang-Feng,
C.K.Shen.
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Ref.
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Genomics, 2001,
73,
238-241.
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PubMed id
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Abstract
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We have cloned a new protein that interacts with the hematopoietic DNA-binding
transcription factor, p45/NF-E2, by screening a human erythroleukemia cell cDNA
library with the yeast two-hybrid approach. Predicted peptide sequence and
chromosomal mapping identified the cloned molecule to be the product of the
human ortholog of the mouse Itch gene, which has been implicated previously in
the regulation of growth and differentiation of erythroid and lymphoid cells.
Transfection experiments indicate that this human ITCH protein can act as a
transcriptional corepressor of p45/NF-E2. Our data provide novel insights into
the functional roles of the mammalian ITCH proteins in the development of
hematopoietic cell lineages.
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