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Title
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The structure of the complex formed by bovine trypsin and bovine pancreatic trypsin inhibitor III. Structure of the anhydro-trypsin-inhibitor complex.
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Authors
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R.Huber,
W.Bode,
D.Kukla,
U.Kohl,
C.A.Ryan.
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Ref.
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Biophys Struct Mech, 1975,
1,
189-201.
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PubMed id
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Abstract
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The structure of the complex between anhydro-trypsin and pancreatic trypsin
inhibitor has been determined by difference Fourier techniques using phases
obtained from the native complex (Huber et al., 1974). It was refined
independently by constrained crystallographic refinement at 1.9 A resolution.
The anhydro-complex has Ser 195 converted to dehydro-alanine. There were no
other significant structural changes. In particular, the high degree of
pyramidalization of the C atom of Lys 15 (I) of the inhibitor component observed
in the native complex in maintained in the anhydro-species.
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