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Title
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Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain.
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Authors
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T.H.Tahirov,
H.Morii,
H.Uedaira,
A.Sarai,
K.Ogata.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 1999,
55,
1345-1347.
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PubMed id
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Abstract
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The R2 subdomain of the mouse c-Myb DNA-binding domain and its V103L mutant have
been crystallized by the vapour-diffusion method using highly concentrated
sodium citrate at pH 6.8 as a precipitant. Using ammonium sulfate as precipitant
in MES buffer only produced crystals for the mutant R2. All crystals are
isomorphous and belong to space group P212121. The unit-cell dimensions for
wild-type R2 crystals grown from sodium citrate precipitant are a = 28.83, b =
40.18, c = 49.23 A. Crystals contain one R2 molecule per asymmetric unit. They
are stable during 3 d exposure to X-rays and diffract to 1.37-1.45 A resolution.
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