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Title
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Preparation of encapsulated proteins dissolved in low viscosity fluids.
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Authors
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M.R.Ehrhardt,
P.F.Flynn,
A.J.Wand.
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Ref.
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J Biomol Nmr, 1999,
14,
75-78.
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PubMed id
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Abstract
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The majority of proteins are too large to be comprehensively examined by
solution NMR methods, primarily because they tumble too slowly in solution. One
potential approach to making the NMR relaxation properties of large proteins
amenable to modern solution NMR techniques is to encapsulate them in a reverse
micelle which is dissolved in a low viscosity fluid. Unfortunately, promising
low viscosity fluids such as the short chain alkanes, supercritical carbon
dioxide, and various halocarbon refrigerants all require the application of
significant pressure to be kept liquefied at room temperature. Here we describe
the design and use of a simple cost effective NMR tube suitable for the
preparation of solutions of proteins encapsulated in reverse micelles dissolved
in such fluids.
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