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Title
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Lack of significant differences in association rates and affinities of antibodies from short-term and long-term responses to hen egg lysozyme.
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Authors
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F.A.Goldbaum,
A.Cauerhff,
C.A.Velikovsky,
A.S.Llera,
M.M.Riottot,
R.J.Poljak.
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Ref.
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J Immunol, 1999,
162,
6040-6045.
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PubMed id
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Abstract
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The affinities (Ka) and association rate constants (kon) of 23 mouse (BALB/c)
anti-lysozyme mAbs obtained after short and prolonged immunizations have been
measured by plasmon resonance techniques. The affinities for the 23 Abs,
measured using their Fab, range from Ka = 1.1 x 10(7) to 1.4 x 10(10) M-1. There
is no significant correlation between time or dose of immunization and affinity
or association rates, indicating no time- or dose-dependent maturation of the
response within the doses and times that were explored. IgMs are produced early
and late in the response, with intrinsic affinities <10(5) M-1. Two
independently derived mAbs, D44.1 (short term) and F10.6.6 (from a longer term
response), result from identical or nearly identical somatic recombination
events of germline gene segments. F10.6.6 has more mutations and a higher
affinity constant (Ka = 1.4 x 10(10) M-1) than D44.1 (Ka = 1.1 x 10(7) M-1).
Although higher affinities may result from an accumulation of mutations, they do
not correlate with the length and dose of immunogenic challenge.
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