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PDBsum entry 6v0r
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Viral protein
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PDB id
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6v0r
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References listed in PDB file
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Key reference
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Title
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Mapping polyclonal antibody responses in non-Human primates vaccinated with HIV env trimer subunit vaccines.
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Authors
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B.Nogal,
M.Bianchi,
C.A.Cottrell,
R.N.Kirchdoerfer,
L.M.Sewall,
H.L.Turner,
F.Zhao,
D.Sok,
D.R.Burton,
L.Hangartner,
A.B.Ward.
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Ref.
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Cell Rep, 2020,
30,
3755.
[DOI no: ]
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PubMed id
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Abstract
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Rational immunogen design aims to focus antibody responses to vulnerable sites
on primary antigens. Given the size of these antigens, there is, however,
potential for eliciting unwanted, off-target responses. Here, we use our
electron microscopy polyclonal epitope mapping approach to describe the antibody
specificities elicited by immunization of non-human primates with soluble HIV
envelope trimers and subsequent repeated viral challenge. An increased diversity
of epitopes recognized and the approach angle by which these antibodies bind
constitute a hallmark of the humoral response in most protected animals. We also
show that fusion peptide-specific antibodies are likely responsible for some
neutralization breadth. Moreover, cryoelectron microscopy (cryo-EM) analysis of
a fully protected animal reveals a high degree of clonality within a subset of
putatively neutralizing antibodies, enabling a detailed molecular description of
the antibody paratope. Our results provide important insights into the immune
response against a vaccine candidate that entered into clinical trials in 2019.
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