PDBsum entry 6qnh

Isomerase

PDB id: 6qnh

Contents

Protein chain

387 a.a.

Metals

_CO

_MG

Waters ×255

References listed in PDB file

Key reference

• Title: Liquid application method for time-Resolved analyses by serial synchrotron crystallography.
• Authors: P.Mehrabi, E.C.Schulz, M.Agthe, S.Horrell, G.Bourenkov, D.Von stetten, J.P.Leimkohl, H.Schikora, T.R.Schneider, A.R.Pearson, F.Tellkamp, R.J.D.Miller.
• Ref.: Nat Methods, 2019, 16, 979-982. [DOI no: 10.1038/s41592-019-0553-1]
• PubMed id: 31527838

Abstract

We introduce a liquid application method for time-resolved analyses (LAMA), an in situ mixing approach for serial crystallography. Picoliter-sized droplets are shot onto chip-mounted protein crystals, achieving near-full ligand occupancy within theoretical diffusion times. We demonstrate proof-of-principle binding of GlcNac to lysozyme, and resolve glucose binding and subsequent ring opening in a time-resolved study of xylose isomerase.