PDBsum entry 6lts

Transcription

PDB id: 6lts

Contents

Protein chains

226 a.a.

1111 a.a.

1486 a.a.

94 a.a.

336 a.a.

DNA/RNA

Metals

_MG ×5

_ZN ×2

References listed in PDB file

Key reference

• Title: Rna extension drives a stepwise displacement of an initiation-Factor structural module in initial transcription.
• Authors: L.Li, V.Molodtsov, W.Lin, R.H.Ebright, Y.Zhang.
• Ref.: Proc Natl Acad Sci U S A, 2020, 117, 5801-5809. [DOI no: 10.1073/pnas.1920747117]
• PubMed id: 32127479

Abstract

All organisms-bacteria, archaea, and eukaryotes-have a transcription initiation factor that contains a structural module that binds within the RNA polymerase (RNAP) active-center cleft and interacts with template-strand single-stranded DNA (ssDNA) in the immediate vicinity of the RNAP active center. This transcription initiation-factor structural module preorganizes template-strand ssDNA to engage the RNAP active center, thereby facilitating binding of initiating nucleotides and enabling transcription initiation from initiating mononucleotides. However, this transcription initiation-factor structural module occupies the path of nascent RNA and thus presumably must be displaced before or during initial transcription. Here, we report four sets of crystal structures of bacterial initially transcribing complexes that demonstrate and define details of stepwise, RNA-extension-driven displacement of the "σ-finger" of the bacterial transcription initiation factor σ. The structures reveal that-for both the primary σ-factor and extracytoplasmic (ECF) σ-factors, and for both 5'-triphosphate RNA and 5'-hydroxy RNA-the "σ-finger" is displaced in stepwise fashion, progressively folding back upon itself, driven by collision with the RNA 5'-end, upon extension of nascent RNA from ∼5 nt to ∼10 nt.