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PDBsum entry 6hu5
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References listed in PDB file
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Key reference
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Title
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Nanobeam precession-Assisted 3d electron diffraction reveals a new polymorph of hen egg-White lysozyme.
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Authors
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A.Lanza,
E.Margheritis,
E.Mugnaioli,
V.Cappello,
G.Garau,
M.Gemmi.
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Ref.
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IUCrJ, 2019,
6,
178-188.
[DOI no: ]
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PubMed id
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Abstract
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Recent advances in 3D electron diffraction have allowed the structure
determination of several model proteins from submicrometric crystals, the
unit-cell parameters and structures of which could be immediately validated by
known models previously obtained by X-ray crystallography. Here, the first new
protein structure determined by 3D electron diffraction data is presented: a
previously unobserved polymorph of hen egg-white lysozyme. This form, with
unit-cell parameters a = 31.9, b = 54.4, c = 71.8 Å, β
= 98.8°, grows as needle-shaped submicrometric crystals simply by vapor
diffusion starting from previously reported crystallization conditions.
Remarkably, the data were collected using a low-dose stepwise experimental setup
consisting of a precession-assisted nanobeam of ∼150 nm, which has never
previously been applied for solving protein structures. The crystal structure
was additionally validated using X-ray synchrotron-radiation sources by both
powder diffraction and single-crystal micro-diffraction. 3D electron diffraction
can be used for the structural characterization of submicrometric macromolecular
crystals and is able to identify novel protein polymorphs that are hardly
visible in conventional X-ray diffraction experiments. Additionally, the
analysis, which was performed on both nanocrystals and microcrystals from the
same crystallization drop, suggests that an integrated view from 3D electron
diffraction and X-ray microfocus diffraction can be applied to obtain insights
into the molecular dynamics during protein crystal growth.
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