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UniProt functional annotation for P05725 | ||
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| UniProt code: P05725. |
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| Organism: | |
Chlamydomonas reinhardtii (Chlamydomonas smithii). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae; CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas. |
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| Function: | |
Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site. |
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| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:11276249, ECO:0000269|PubMed:12758074, ECO:0000269|PubMed:15518550}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:11276249, ECO:0000269|PubMed:12758074, ECO:0000269|PubMed:15518550}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:11276249, ECO:0000269|PubMed:12758074, ECO:0000269|PubMed:15518550}; Name=Ni(2+); Xref=ChEBI:CHEBI:49786; Evidence={ECO:0000269|PubMed:11276249, ECO:0000269|PubMed:12758074, ECO:0000269|PubMed:15518550}; Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:11276249, ECO:0000269|PubMed:12758074, ECO:0000269|PubMed:15518550}; Note=Binds 3 Mg(2+) ions per homodimer. The enzyme can also utilize Mn(2+) or Co(2+), but has lower cleavage activity with Ni(2+) or Zn(2+). Ca(2+) Co(2+) give no enzyme activity. {ECO:0000269|PubMed:11276249, ECO:0000269|PubMed:12758074, ECO:0000269|PubMed:15518550}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:11276249, ECO:0000269|PubMed:12419232, ECO:0000269|PubMed:12758074, ECO:0000269|PubMed:15313605, ECO:0000269|PubMed:15518550, ECO:0000269|PubMed:16971456, ECO:0000269|PubMed:17452357}. |
| Subcellular location: | |
Plastid, chloroplast. |
| Similarity: | |
Belongs to the LAGLIDADG endonuclease family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.