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PDBsum entry 6f3k
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Peptide binding protein
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PDB id
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6f3k
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References listed in PDB file
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Key reference
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Title
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Integrated nmr and cryo-Em atomic-Resolution structure determination of a half-Megadalton enzyme complex.
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Authors
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D.F.Gauto,
L.F.Estrozi,
C.D.Schwieters,
G.Effantin,
P.Macek,
R.Sounier,
A.C.Sivertsen,
E.Schmidt,
R.Kerfah,
G.Mas,
J.P.Colletier,
P.Güntert,
A.Favier,
G.Schoehn,
P.Schanda,
J.Boisbouvier.
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Ref.
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Nat Commun, 2019,
10,
2697.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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Atomic-resolution structure determination is crucial for understanding protein
function. Cryo-EM and NMR spectroscopy both provide structural information, but
currently cryo-EM does not routinely give access to atomic-level structural
data, and, generally, NMR structure determination is restricted to small
(<30 kDa) proteins. We introduce an integrated structure determination
approach that simultaneously uses NMR and EM data to overcome the limits of each
of these methods. The approach enables structure determination of the 468 kDa
large dodecameric aminopeptidase TET2 to a precision and accuracy below 1 Å
by combining secondary-structure information obtained from near-complete
magic-angle-spinning NMR assignments of the 39 kDa-large subunits, distance
restraints from backbone amides and ILV methyl groups, and a 4.1 Å resolution
EM map. The resulting structure exceeds current standards of NMR and EM
structure determination in terms of molecular weight and precision. Importantly,
the approach is successful even in cases where only medium-resolution cryo-EM
data are available.
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