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PDBsum entry 6erh
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DNA binding protein
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PDB id
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6erh
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References listed in PDB file
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Key reference
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Title
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Xlf and aplf bind ku80 at two remote sites to ensure DNA repair by non-Homologous end joining.
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Authors
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C.Nemoz,
V.Ropars,
P.Frit,
A.Gontier,
P.Drevet,
J.Yu,
R.Guerois,
A.Pitois,
A.Comte,
C.Delteil,
N.Barboule,
P.Legrand,
S.Baconnais,
Y.Yin,
S.Tadi,
E.Barbet-Massin,
I.Berger,
E.Le cam,
M.Modesti,
E.Rothenberg,
P.Calsou,
J.B.Charbonnier.
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Ref.
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Nat Struct Mol Biol, 2018,
25,
971-980.
[DOI no: ]
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PubMed id
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Abstract
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The Ku70-Ku80 (Ku) heterodimer binds rapidly and tightly to the ends of DNA
double-strand breaks and recruits factors of the non-homologous end-joining
(NHEJ) repair pathway through molecular interactions that remain unclear. We
have determined crystal structures of the Ku-binding motifs (KBM) of the NHEJ
proteins APLF (A-KBM) and XLF (X-KBM) bound to a Ku-DNA complex. The two KBM
motifs bind remote sites of the Ku80 α/β domain. The X-KBM occupies an
internal pocket formed by an unprecedented large outward rotation of the Ku80
α/β domain. We observe independent recruitment of the APLF-interacting protein
XRCC4 and of XLF to laser-irradiated sites via binding of A- and X-KBMs,
respectively, to Ku80. Finally, we show that mutation of the X-KBM and A-KBM
binding sites in Ku80 compromises both the efficiency and accuracy of end
joining and cellular radiosensitivity. A- and X-KBMs may represent two initial
anchor points to build the intricate interaction network required for NHEJ.
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