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PDBsum entry 6cla
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References listed in PDB file
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Key reference
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Title
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Analysis of global and site-Specific radiation damage in cryo-Em.
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Authors
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J.Hattne,
D.Shi,
C.Glynn,
C.T.Zee,
M.Gallagher-Jones,
M.W.Martynowycz,
J.A.Rodriguez,
T.Gonen.
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Ref.
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Structure, 2018,
26,
759.
[DOI no: ]
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PubMed id
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Abstract
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Micro-crystal electron diffraction (MicroED) combines the efficiency of electron
scattering with diffraction to allow structure determination from nano-sized
crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to
solve structures of a diverse set of biomolecules and materials, in some cases
to sub-atomic resolution. However, little is known about the damaging effects of
the electron beam on samples during such measurements. We assess global and
site-specific damage from electron radiation on nanocrystals of proteinase K and
of a prion hepta-peptide and find that the dynamics of electron-induced damage
follow well-established trends observed in X-ray crystallography. Metal ions are
perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated
while the diffracted intensities decay exponentially with increasing exposure.
A better understanding of radiation damage in MicroED improves our assessment
and processing of all types of cryo-EM data.
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