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PDBsum entry 6b6h
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Transcription/transferase/DNA/RNA
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PDB id
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6b6h
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Contents |
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230 a.a.
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1340 a.a.
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1337 a.a.
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79 a.a.
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497 a.a.
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201 a.a.
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75 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis of bacterial transcription activation.
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Authors
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B.Liu,
C.Hong,
R.K.Huang,
Z.Yu,
T.A.Steitz.
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Ref.
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Science, 2017,
358,
947-951.
[DOI no: ]
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PubMed id
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Abstract
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In bacteria, the activation of gene transcription at many promoters is simple
and only involves a single activator. The cyclic adenosine 3',5'-monophosphate
receptor protein (CAP), a classic activator, is able to activate transcription
independently through two different mechanisms. Understanding the class I
mechanism requires an intact transcription activation complex (TAC) structure at
a high resolution. Here we report a high-resolution cryo-electron microscopy
structure of an intactEscherichia coliclass I TAC containing a CAP dimer,
a σ70-RNA polymerase (RNAP) holoenzyme, a complete class I
CAP-dependent promoter DNA, and a de novo synthesized RNA oligonucleotide. The
structure shows how CAP wraps the upstream DNA and how the interactions recruit
RNAP. Our study provides a structural basis for understanding how activators
activate transcription through the class I recruitment mechanism.
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