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PDBsum entry 6mh6
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PDB id:
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Hydrolase
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Title:
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High-viscosity injector-based pink beam serial crystallography of micro-crystals at a synchrotron radiation source.
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Structure:
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Proteinase k. Chain: a. Synonym: endopeptidase k,tritirachium alkaline proteinase. Engineered: yes
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Source:
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Synthetic: yes. Parengyodontium album. Organism_taxid: 37998
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Resolution:
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1.80Å
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R-factor:
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0.134
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R-free:
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0.175
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Authors:
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J.M.Martin-Garcia,L.Zhu,D.Mendez,M.Lee,E.Chun,C.Li,H.Hu, G.Subramanian,D.Kissick,C.Ogata,R.Henning,A.Ishchenko,Z.Dobson, S.Zhan,U.Weierstall,J.C.H.Spence,P.Fromme,N.A.Zatsepin, R.F.Fischetti,V.Cherezov,W.Liu
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Key ref:
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J.M.Martin-Garcia
et al.
(2019).
High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source.
IUCrJ,
6,
412-425.
PubMed id:
DOI:
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Date:
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17-Sep-18
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Release date:
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24-Apr-19
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PROCHECK
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Headers
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References
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P06873
(PRTK_PARAQ) -
Proteinase K from Parengyodontium album
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Seq:
Struc:
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384 a.a.
279 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.3.4.21.64
- peptidase K.
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Reaction:
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Hydrolysis of keratin and of other proteins, with subtilisin-like specificity. Hydrolyzes peptides amides.
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DOI no:
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IUCrJ
6:412-425
(2019)
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PubMed id:
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High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source.
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J.M.Martin-Garcia,
L.Zhu,
D.Mendez,
M.Y.Lee,
E.Chun,
C.Li,
H.Hu,
G.Subramanian,
D.Kissick,
C.Ogata,
R.Henning,
A.Ishchenko,
Z.Dobson,
S.Zhang,
U.Weierstall,
J.C.H.Spence,
P.Fromme,
N.A.Zatsepin,
R.F.Fischetti,
V.Cherezov,
W.Liu.
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ABSTRACT
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Since the first successful serial crystallography (SX) experiment at a
synchrotron radiation source, the popularity of this approach has continued to
grow showing that third-generation synchrotrons can be viable alternatives to
scarce X-ray free-electron laser sources. Synchrotron radiation flux may be
increased ∼100 times by a moderate increase in the bandwidth ('pink beam'
conditions) at some cost to data analysis complexity. Here, we report the first
high-viscosity injector-based pink-beam SX experiments. The structures of
proteinase K (PK) and A2A adenosine receptor (A2AAR) were
determined to resolutions of 1.8 and 4.2 Å using 4 and 24 consecutive
100 ps X-ray pulse exposures, respectively. Strong PK data were processed
using existing Laue approaches, while weaker A2AAR data required an
alternative data-processing strategy. This demonstration of the feasibility
presents new opportunities for time-resolved experiments with microcrystals to
study structural changes in real time at pink-beam synchrotron beamlines
worldwide.
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