PDBsum entry 6jbp

Plant protein

PDB id: 6jbp

Contents

Protein chain

179 a.a.

Waters ×88

PDB id:

6jbp

Name:

Plant protein

Title:

Structure of mp-4 from mucuna pruriens at 2.22 angstroms

Structure:

Kunitz-type trypsin inhibitor-like 2 protein. Chain: b

Source:

Mucuna pruriens. Velvet bean. Organism_taxid: 157652

Resolution:

2.22Å R-factor: 0.181 R-free: 0.220

Authors:

A.Jain,M.Shikhi,A.Kumar,A.Kumar,D.T.Nair,D.M.Salunke

Key ref:

A.Jain et al. (2020). The structure of MP-4 from Mucuna pruriens at 2.22 Å resolution. Acta Crystallogr F Struct Biol Commun, 76, 47-57. PubMed id: 32039885 DOI: 10.1107/S2053230X20000199

Date:

26-Jan-19 Release date: 29-Jan-20

Protein chain

A0A371E4L6  (A0A371E4L6_MUCPR) -  Kunitz-type trypsin inhibitor-like 2 protein (Fragment) from Mucuna pruriens

Seq: 216 a.a.

Struc: 179 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1107/S2053230X20000199

Acta Crystallogr F Struct Biol Commun 76:47-57 (2020)

PubMed id: 32039885

The structure of MP-4 from Mucuna pruriens at 2.22 Å resolution.

A.Jain, A.Kumar, M.Shikhi, A.Kumar, D.T.Nair, D.M.Salunke.

ABSTRACT

The structure of the MP-4 protein was previously determined at a resolution of 2.8 Å. Owing to the unavailability of gene-sequence information at the time, the side-chain assignment was carried out on the basis of a partial sequence available through Edman degradation, sequence homology to orthologs and electron density. The structure of MP-4 has now been determined at a higher resolution (2.22 Å) in another space group and all of the structural inferences that were presented in the previous report of the structure were validated. In addition, the present data allowed an improved assignment of side chains and enabled further analysis of the MP-4 structure, and the accuracy of the assignment was confirmed by the recently available gene sequence. The study reinforces the traditional concept that conservative interpretations of relatively low-resolution structures remain correct even with the availability of high-resolution data.