PDBsum entry 6aqr

Hydrolase

PDB id: 6aqr

Contents

Protein chains

442 a.a.

91 a.a.

87 a.a.

76 a.a.

88 a.a.

Metals

_ZN ×8

Waters ×264

PDB id:

6aqr

Name:

Hydrolase

Title:

Saga dub module ubp8(c146a)/sgf11/sus1/sgf73 bound to monoubiquitin

Structure:

Ubiquitin carboxyl-terminal hydrolase 8. Chain: a. Synonym: deubiquitinating enzyme 8,ubiquitin thioesterase 8, ubiquitin-specific-processing protease 8. Engineered: yes. Mutation: yes. Transcription and mRNA export factor sus1. Chain: b. Engineered: yes.

Source:

Saccharomyces cerevisiae s288c. Baker's yeast. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: ubp8, ymr223w, ym9959.05. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: sus1, ybr111w-a. Gene: sgf11, ypl047w.

Resolution:

2.10Å R-factor: 0.204 R-free: 0.249

Authors:

M.E.Morrow,M.T.Morgan,C.Wolberger

Key ref:

m.e.morrow et al. (2018). Active site alanine mutations convert deubiquitinases into high-affinity ubiquitin-binding proteins. EMBO Rep., 0, . PubMed id: 30150323

Date:

21-Aug-17 Release date: 04-Jul-18

Protein chain

P50102  (UBP8_YEAST) -  Ubiquitin carboxyl-terminal hydrolase 8 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 471 a.a.

Struc: 442 a.a.*

Protein chain

Q6WNK7  (SUS1_YEAST) -  SAGA complex subunit SUS1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 96 a.a.

Struc: 91 a.a.

Protein chain

Q03067  (SGF11_YEAST) -  SAGA complex subunit SGF11 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 99 a.a.

Struc: 87 a.a.

Protein chain

P0CG48  (UBC_HUMAN) -  Polyubiquitin-C from Homo sapiens

Seq: 685 a.a.

Struc: 76 a.a.

Protein chain

P53165  (SGF73_YEAST) -  SAGA complex subunit SGF73 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 657 a.a.

Struc: 88 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

EMBO Rep. 0: (2018)

PubMed id: 30150323

Active site alanine mutations convert deubiquitinases into high-affinity ubiquitin-binding proteins.

m.e.morrow, m.t.morgan, c.wolberger.

ABSTRACT

No abstract given.