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PDBsum entry 5wjh
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References listed in PDB file
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Key reference
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Title
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Using sound pulses to solve the crystal-Harvesting bottleneck.
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Authors
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Y.N.Samara,
H.M.Brennan,
L.Mccarthy,
M.T.Bollard,
D.Laspina,
J.M.Wlodek,
S.L.Campos,
R.Natarajan,
K.Gofron,
S.Mcsweeney,
A.S.Soares,
L.Leroy.
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Ref.
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Acta Crystallogr D Struct Biol, 2018,
74,
986-999.
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PubMed id
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Abstract
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Crystal harvesting has proven to be difficult to automate and remains the
rate-limiting step for many structure-determination and high-throughput
screening projects. This has resulted in crystals being prepared more rapidly
than they can be harvested for X-ray data collection. Fourth-generation
synchrotrons will support extraordinarily rapid rates of data acquisition,
putting further pressure on the crystal-harvesting bottleneck. Here, a simple
solution is reported in which crystals can be acoustically harvested from
slightly modified MiTeGen In Situ-1 crystallization plates. This technique uses
an acoustic pulse to eject each crystal out of its crystallization well, through
a short air column and onto a micro-mesh (improving on previous work, which
required separately grown crystals to be transferred before harvesting).
Crystals can be individually harvested or can be serially combined with a
chemical library such as a fragment library.
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