PDBsum entry 5whw

Hydrolase

PDB id: 5whw

Contents

Protein chain

279 a.a.

Ligands

NO3

EDO ×3

BCN

Metals

_CA ×2

Waters ×427

References listed in PDB file

Key reference

• Title: Using sound pulses to solve the crystal-Harvesting bottleneck.
• Authors: Y.N.Samara, H.M.Brennan, L.Mccarthy, M.T.Bollard, D.Laspina, J.M.Wlodek, S.L.Campos, R.Natarajan, K.Gofron, S.Mcsweeney, A.S.Soares, L.Leroy.
• Ref.: Acta Crystallogr D Struct Biol, 2018, 74, 986-999.
• PubMed id: 30289409

Abstract

Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ-1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library.