PDBsum entry 5uzm

RNA binding protein

PDB id: 5uzm

Contents

Protein chains

90 a.a.

Waters ×127

References listed in PDB file

Key reference

• Title: The drosophila hnrnp f/h homolog glorund uses two distinct RNA-Binding modes to diversify target recognition.
• Authors: J.V.Tamayo, T.Teramoto, S.Chatterjee, T.M.Hall, E.R.Gavis.
• Ref.: Cell Rep, 2017, 19, 150-161.
• PubMed id: 28380354

Abstract

The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3' untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo's RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo's functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.