PDBsum entry 5uzm

RNA binding protein

PDB id: 5uzm

Contents

Protein chains

90 a.a.

Waters ×127

PDB id:

5uzm

Name:

RNA binding protein

Title:

Crystal structure of glorund qrrm2 domain

Structure:

At27789p. Chain: a, b. Fragment: residues 142-234. Synonym: glorund,isoform a,isoform b. Engineered: yes

Source:

Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: glo, cg6946, dmel_cg6946. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.55Å R-factor: 0.196 R-free: 0.239

Authors:

T.Teramoto,T.M.T.Hall

Key ref:

J.V.Tamayo et al. (2017). The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition. Cell Rep, 19, 150-161. PubMed id: 28380354

Date:

27-Feb-17 Release date: 22-Mar-17

Protein chains

Q9VGH5  (Q9VGH5_DROME) -  AT27789p from Drosophila melanogaster

Seq: 586 a.a.

Struc: 90 a.a.

Enzyme reactions

• Enzyme class: E.C.?

Cell Rep 19:150-161 (2017)

PubMed id: 28380354

The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition.

J.V.Tamayo, T.Teramoto, S.Chatterjee, T.M.Hall, E.R.Gavis.

ABSTRACT

The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3' untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo's RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo's functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins.