UniProt functional annotation for Q9STE8



	UniProt functional annotation for Q9STE8

UniProt code: Q9STE8.

Organism: Arabidopsis thaliana (Mouse-ear cress).

Taxonomy: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.

Function: Essential protein. Mediates the insertion of proteins targeted to the outer membrane of chloroplasts (By similarity). Required for the import of protein precursors into chloroplasts. Forms the voltage-dependent preprotein translocation channels (hydrophilic beta barrel) of the TOC complex in the chloroplastic outer membrane. {ECO:0000250, ECO:0000269|PubMed:15908591}.

Subunit: Part of the TOC core complex that includes a protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP- binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursors such as prSS (precursor of the RuBisCO small subunit) also interact with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). TOC75-3 interacts with TOC34/OEP34, TOC159/TOC86, TOC132 and TOC120 (PubMed:15090618). Interacts with SP1 (PubMed:23118188). Interacts with TIC236 (PubMed:30464337). {ECO:0000269|PubMed:15090618, ECO:0000269|PubMed:23118188, ECO:0000269|PubMed:30464337}.

Subcellular location: Plastid, chloroplast outer membrane {ECO:0000269|PubMed:12766230, ECO:0000269|PubMed:15090618}; Multi-pass membrane protein {ECO:0000269|PubMed:12766230, ECO:0000269|PubMed:15090618}.

Tissue specificity: Mostly expressed in young and actively dividing photosynthetic tissues and, to a lower extent, in old leaves and roots. Particularly low levels in leaves after etiolation. {ECO:0000269|PubMed:15908591}.

Developmental stage: Expressed predominantly during the early stages of plant growth, peaking at three weeks after germination (at protein level). {ECO:0000269|PubMed:15090618}.

Induction: Up-regulated by CIA2 in leaves. {ECO:0000269|PubMed:11549763}.

Domain: Contains 3 N-terminal periplasmic polypeptide transport- associated (POTRA) domains which may serve as a binding site for the preprotein in the chloroplast intermembrane space, and provide a chaperone-like activity to prevent misfolding or aggregation in the intermembrane space (Probable). Transmembrane regions consist mainly of membrane-spanning sided beta-sheets, which are not predicted by sequence analysis tools (Probable). {ECO:0000305|PubMed:15908591, ECO:0000305|PubMed:28559331}.

Disruption phenotype: Plants have an embryo development arrested at the two cells stage. {ECO:0000269|PubMed:15908591}.

Similarity: Belongs to the TOC75 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Arabidopsis thaliana (Mouse-ear cress).
Taxonomy:		Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.



Function:		Essential protein. Mediates the insertion of proteins targeted to the outer membrane of chloroplasts (By similarity). Required for the import of protein precursors into chloroplasts. Forms the voltage-dependent preprotein translocation channels (hydrophilic beta barrel) of the TOC complex in the chloroplastic outer membrane. {ECO:0000250, ECO:0000269\|PubMed:15908591}.


Subunit:		Part of the TOC core complex that includes a protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP- binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursors such as prSS (precursor of the RuBisCO small subunit) also interact with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). TOC75-3 interacts with TOC34/OEP34, TOC159/TOC86, TOC132 and TOC120 (PubMed:15090618). Interacts with SP1 (PubMed:23118188). Interacts with TIC236 (PubMed:30464337). {ECO:0000269\|PubMed:15090618, ECO:0000269\|PubMed:23118188, ECO:0000269\|PubMed:30464337}.
Subcellular location:		Plastid, chloroplast outer membrane {ECO:0000269\|PubMed:12766230, ECO:0000269\|PubMed:15090618}; Multi-pass membrane protein {ECO:0000269\|PubMed:12766230, ECO:0000269\|PubMed:15090618}.
Tissue specificity:		Mostly expressed in young and actively dividing photosynthetic tissues and, to a lower extent, in old leaves and roots. Particularly low levels in leaves after etiolation. {ECO:0000269\|PubMed:15908591}.
Developmental stage:		Expressed predominantly during the early stages of plant growth, peaking at three weeks after germination (at protein level). {ECO:0000269\|PubMed:15090618}.
Induction:		Up-regulated by CIA2 in leaves. {ECO:0000269\|PubMed:11549763}.
Domain:		Contains 3 N-terminal periplasmic polypeptide transport- associated (POTRA) domains which may serve as a binding site for the preprotein in the chloroplast intermembrane space, and provide a chaperone-like activity to prevent misfolding or aggregation in the intermembrane space (Probable). Transmembrane regions consist mainly of membrane-spanning sided beta-sheets, which are not predicted by sequence analysis tools (Probable). {ECO:0000305\|PubMed:15908591, ECO:0000305\|PubMed:28559331}.
Disruption phenotype:		Plants have an embryo development arrested at the two cells stage. {ECO:0000269\|PubMed:15908591}.
Similarity:		Belongs to the TOC75 family. {ECO:0000305}.