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PDBsum entry 5tdr
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Transcription
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PDB id
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5tdr
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References listed in PDB file
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Key reference
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Title
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Structural insight into recognition of methylated histone h3k4 by set3.
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Authors
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J.Gatchalian,
M.Ali,
F.H.Andrews,
Y.Zhang,
A.S.Barrett,
T.G.Kutateladze.
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Ref.
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J Mol Biol, 2017,
429,
2066-2074.
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PubMed id
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Abstract
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The plant homeodomain (PHD) finger of Set3 binds methylated lysine 4 of histone
H3 in vitro and in vivo; however, precise selectivity of this domain has not
been fully characterized. Here, we explore the determinants of methyllysine
recognition by the PHD fingers of Set3 and its orthologs. We use X-ray
crystallographic and spectroscopic approaches to show that the Set3 PHD finger
binds di- and trimethylated states of H3K4 with comparable affinities and
employs similar molecular mechanisms to form complexes with either mark.
Composition of the methyllysine-binding pocket plays an essential role in
determining the selectivity of the PHD fingers. The finding that the
histone-binding activity is not conserved in the PHD finger of Set4 suggests
different functions for the Set3 and Set4 paralogs.
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