spacer
spacer

PDBsum entry 5t3n
Go to PDB code: 
protein ligands metals Protein-protein interface(s) links
Transferase PDB id
5t3n

 

 

 

 

Loading ...

 
JSmol PyMol  
Contents
Protein chains
153 a.a.
Ligands
75G ×2
Metals
IOD ×5
Waters ×10
PDB id:
5t3n
Name: Transferase
Title: Sp-2cl-camps bound to pkar cbd2
Structure: Camp-dependent protein kinase regulatory subunit. Chain: a, b. Engineered: yes. Other_details: n-terminal hexahistidine tag
Source: Plasmodium falciparum (isolate 3d7). Organism_taxid: 36329. Strain: isolate 3d7. Gene: pkar, pf3d7_1223100, pfl1110c. Expressed in: escherichia coli. Expression_system_taxid: 83333. Expression_system_variant: de3.
Resolution:
2.40Å     R-factor:   0.254     R-free:   0.307
Authors: D.R.Littler,P.Gilson
Key ref: D.R.Littler et al. (2016). Disrupting the Allosteric Interaction between the Plasmodium falciparum cAMP-dependent Kinase and Its Regulatory Subunit. J Biol Chem, 291, 25375-25386. PubMed id: 27738107 DOI: 10.1074/jbc.M116.750174
Date:
26-Aug-16     Release date:   12-Oct-16    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q7KQK0  (Q7KQK0_PLAF7) -  cAMP-dependent protein kinase regulatory subunit from Plasmodium falciparum (isolate 3D7)
Seq:
Struc: 		441 a.a.
153 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 10 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.2.7.11.11  - cAMP-dependent protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H+
L-seryl-[protein]
 + ATP
 = O-phospho-L-seryl-[protein]
Bound ligand (Het Group name = 75G)
matches with 72.41% similarity 		+ ADP
 + H(+)
L-threonyl-[protein]
 + ATP
 = O-phospho-L-threonyl-[protein]
Bound ligand (Het Group name = 75G)
matches with 72.41% similarity 		+ ADP
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
DOI no: 10.1074/jbc.M116.750174 J Biol Chem 291:25375-25386 (2016)
PubMed id: 27738107  
 
 
Disrupting the Allosteric Interaction between the Plasmodium falciparum cAMP-dependent Kinase and Its Regulatory Subunit.
D.R.Littler, H.E.Bullen, K.L.Harvey, T.Beddoe, B.S.Crabb, J.Rossjohn, P.R.Gilson.
 
  ABSTRACT  
 
No abstract given.

 

 

spacer
spacer