PDBsum entry 5t0m

Transferase

PDB id: 5t0m

Contents

Protein chains

260 a.a.

Ligands

THR-LYS-GLN-THR-ALA-ARG-NLE-SER-THR-GLY ×2

SAM ×2

SAH

Metals

_ZN ×8

Waters ×352

PDB id:

5t0m

Name:

Transferase

Title:

A histone h3k9m mutation traps histone methyltransferase clr4 to prevent heterochromatin spreading

Structure:

Histone-lysine n-methyltransferase ehmt2. Chain: a, b. Fragment: unp residues 879-1159. Synonym: euchromatic histone-lysine n-methyltransferase 2,hla-b- associated transcript 8,histone h3-k9 methyltransferase 3,h3-k9- hmtase 3,lysine n-methyltransferase 1c,protein g9a. Engineered: yes. Thr-lys-gln-thr-ala-arg-nle-ser-thr-gly. Chain: p, c.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ehmt2, bat8, c6orf30, g9a, kmt1c, ng36. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 9606

Resolution:

1.90Å R-factor: 0.186 R-free: 0.224

Authors:

K.Xu,L.Tong

Key ref:

C.M.Shan et al. (2016). A histone h3k9m mutation traps histone methyltransfer clr4 to prevent heterochromatin spreading.. Elife, 5, . PubMed id: 27648579

Date:

16-Aug-16 Release date: 05-Oct-16

Protein chains

Q96KQ7  (EHMT2_HUMAN) -  Histone-lysine N-methyltransferase EHMT2 from Homo sapiens

Seq: 1210 a.a.

Struc: 260 a.a.

Enzyme reactions

• Enzyme class 2: E.C.2.1.1.- - ?????
• Enzyme class 3: E.C.2.1.1.367 - [histone H3]-lysine(9) N-methyltransferase.
• Reaction: L-lysyl₉-[histone H3] + S-adenosyl-L-methionine = N₆-methyl-L- lysyl₉-[histone H3] + S-adenosyl-L-homocysteine + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site