PDBsum entry 5swl

Hydrolase

PDB id: 5swl

Contents

Protein chains

336 a.a.

Ligands

SO4 ×2

Waters ×106

PDB id:

5swl

Name:

Hydrolase

Title:

Crystal structure of atpase delta1-79 spa47 e188a

Structure:

Probable atp synthase spal/mxib. Chain: a, b. Fragment: unp residues 80-430. Synonym: spa47. Engineered: yes. Mutation: yes

Source:

Shigella flexneri. Organism_taxid: 623. Strain: 2457t. Gene: spal, mxib, spa47, cp0149. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.70Å R-factor: 0.203 R-free: 0.259

Authors:

Y.Morales,S.J.Johnson,J.L.Burgess,R.A.Burgess,N.E.Dickenson

Key ref:

J.L.Burgess et al. (2016). Structural and Biochemical Characterization of Spa47 Provides Mechanistic Insight into Type III Secretion System ATPase Activation and Shigella Virulence Regulation. J Biol Chem, 291, 25837-25852. PubMed id: 27770024 DOI: 10.1074/jbc.M116.755256

Date:

08-Aug-16 Release date: 02-Nov-16

Protein chains

P0A1C1  (SPAL_SHIFL) -  Type 3 secretion system ATPase from Shigella flexneri

Seq: 430 a.a.

Struc: 336 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.7.4.2.8 - protein-secreting ATPase.
• Reaction: ATP + H2O + cellular proteinSide 1 = ADP + phosphate + cellular proteinSide 2

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1074/jbc.M116.755256

J Biol Chem 291:25837-25852 (2016)

PubMed id: 27770024

Structural and Biochemical Characterization of Spa47 Provides Mechanistic Insight into Type III Secretion System ATPase Activation and Shigella Virulence Regulation.

J.L.Burgess, R.A.Burgess, Y.Morales, J.M.Bouvang, S.J.Johnson, N.E.Dickenson.

ABSTRACT

No abstract given.