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PDBsum entry 5swj
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PDB id:
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Hydrolase
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Title:
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Crystal structure of atpase delta1-79 spa47
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Structure:
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Probable atp synthase spal/mxib. Chain: a, b. Fragment: unp residues 80-430. Synonym: spa47. Engineered: yes
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Source:
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Shigella flexneri. Organism_taxid: 623. Strain: 2457t. Gene: spal, mxib, spa47, cp0149. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.40Å
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R-factor:
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0.198
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R-free:
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0.237
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Authors:
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Y.Morales,S.J.Johnson,J.L.Burgess,R.A.Burgess,N.E.Dickenson
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Key ref:
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J.L.Burgess
et al.
(2016).
Structural and Biochemical Characterization of Spa47 Provides Mechanistic Insight into Type III Secretion System ATPase Activation and Shigella Virulence Regulation.
J Biol Chem,
291,
25837-25852.
PubMed id:
DOI:
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Date:
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08-Aug-16
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Release date:
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02-Nov-16
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PROCHECK
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Headers
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References
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P0A1C1
(SPAL_SHIFL) -
Type 3 secretion system ATPase from Shigella flexneri
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Seq:
Struc:
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430 a.a.
337 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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Enzyme class:
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E.C.7.4.2.8
- protein-secreting ATPase.
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Reaction:
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ATP + H2O + cellular proteinSide 1 = ADP + phosphate + cellular proteinSide 2
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ATP
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+
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H2O
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+
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cellular proteinSide 1
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=
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ADP
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+
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phosphate
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+
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cellular proteinSide 2
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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DOI no:
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J Biol Chem
291:25837-25852
(2016)
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PubMed id:
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Structural and Biochemical Characterization of Spa47 Provides Mechanistic Insight into Type III Secretion System ATPase Activation and Shigella Virulence Regulation.
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J.L.Burgess,
R.A.Burgess,
Y.Morales,
J.M.Bouvang,
S.J.Johnson,
N.E.Dickenson.
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ABSTRACT
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