PDBsum entry 5o4c

Membrane protein

PDB id: 5o4c

Contents

Protein chains

332 a.a.

258 a.a.

273 a.a.

323 a.a.

Ligands

HEC ×4

DGA

SO4 ×12

LDA ×4

HTO ×2

BCB ×4

BPB ×2

MQ7

NS5

Metals

FE2

Waters ×13

PDB id:

5o4c

Name:

Membrane protein

Title:

From macrocrystals to microcrystals: a strategy for membrane protein serial crystallography

Structure:

Photosynthetic reaction center cytochromE C subunit. Chain: c. Synonym: cytochrome c558/c559. Reaction center protein h chain. Chain: h. Synonym: photosynthetic reaction center h subunit. Reaction center protein l chain. Chain: l. Synonym: photosynthetic reaction center l subunit.

Source:

Blastochloris viridis. Organism_taxid: 1079. Organism_taxid: 1079

Resolution:

2.80Å R-factor: 0.167 R-free: 0.196

Authors:

R.Dods,P.Baath,G.Branden,R.Neutze

Key ref:

R.Dods et al. (2017). From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography. Structure, 25, 1461. PubMed id: 28781082 DOI: 10.1016/j.str.2017.07.002

Date:

28-May-17 Release date: 16-Aug-17

Protein chain

P07173  (CYCR_BLAVI) -  Photosynthetic reaction center cytochrome c subunit from Blastochloris viridis

Seq: 356 a.a.

Struc: 332 a.a.

Protein chain

P06008  (RCEH_BLAVI) -  Reaction center protein H chain from Blastochloris viridis

Seq: 258 a.a.

Struc: 258 a.a.*

Protein chain

P06009  (RCEL_BLAVI) -  Reaction center protein L chain from Blastochloris viridis

Seq: 274 a.a.

Struc: 273 a.a.

Protein chain

P06010  (RCEM_BLAVI) -  Reaction center protein M chain from Blastochloris viridis

Seq: 324 a.a.

Struc: 323 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DOI no: 10.1016/j.str.2017.07.002

Structure 25:1461 (2017)

PubMed id: 28781082

From Macrocrystals to Microcrystals: A Strategy for Membrane Protein Serial Crystallography.

R.Dods, P.Båth, D.Arnlund, K.R.Beyerlein, G.Nelson, M.Liang, R.Harimoorthy, P.Berntsen, E.Malmerberg, L.Johansson, R.Andersson, R.Bosman, S.Carbajo, E.Claesson, C.E.Conrad, P.Dahl, G.Hammarin, M.S.Hunter, C.Li, S.Lisova, D.Milathianaki, J.Robinson, C.Safari, A.Sharma, G.Williams, C.Wickstrand, O.Yefanov, J.Davidsson, D.P.DePonte, A.Barty, G.Brändén, R.Neutze.

ABSTRACT

Serial protein crystallography was developed at X-ray free-electron lasers (XFELs) and is now also being applied at storage ring facilities. Robust strategies for the growth and optimization of microcrystals are needed to advance the field. Here we illustrate a generic strategy for recovering high-density homogeneous samples of microcrystals starting from conditions known to yield large (macro) crystals of the photosynthetic reaction center of Blastochloris viridis (RC_vir). We first crushed these crystals prior to multiple rounds of microseeding. Each cycle of microseeding facilitated improvements in the RC_virserial femtosecond crystallography (SFX) structure from 3.3-Å to 2.4-Å resolution. This approach may allow known crystallization conditions for other proteins to be adapted to exploit novel scientific opportunities created by serial crystallography.