PDBsum entry 5nce

Plant protein

PDB id: 5nce

Contents

Protein chain

50 a.a.

PDB id:

5nce

Name:

Plant protein

Title:

Structure of psdef1 defensin from pinus sylvestris

Structure:

Defensin-1. Chain: a. Fragment: unp residues 34-83. Engineered: yes

Source:

Pinus sylvestris. Scots pine. Organism_taxid: 3349. Gene: def1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta-gami plyss b.

NMR struc:

15 models

Authors:

B.I.Khairutdinov,E.A.Ermakova,E.K.Bessolitsyna,Y.Y.Toporkova, N.B.Tarasova,V.Kovaleva,Y.F.Zuev,I.V.Nesmelova

Key ref:

B.I.Khairutdinov et al. (2017). NMR structure, conformational dynamics, and biological activity of PsDef1 defensin from Pinus sylvestris. Biochim Biophys Acta, 1865, 1085-1094. PubMed id: 28528214 DOI: 10.1016/j.bbapap.2017.05.012

Date:

03-Mar-17 Release date: 07-Jun-17

Protein chain

A4L7R7  (DEF1_PINSY) -  Defensin-1 from Pinus sylvestris

Seq: 83 a.a.

Struc: 50 a.a.

DOI no: 10.1016/j.bbapap.2017.05.012

Biochim Biophys Acta 1865:1085-1094 (2017)

PubMed id: 28528214

NMR structure, conformational dynamics, and biological activity of PsDef1 defensin from Pinus sylvestris.

B.I.Khairutdinov, E.A.Ermakova, Y.M.Yusypovych, E.K.Bessolicina, N.B.Tarasova, Y.Y.Toporkova, V.Kovaleva, Y.F.Zuev, I.V.Nesmelova.

ABSTRACT

Plants have developed a complex defense response system against pests and pathogens. Defensins, produced by plants as part of their innate immune response, form the family of small, basic, cysteine-rich proteins with activity primarily directed against fungal pathogens. In addition, plant defensins can show antibacterial activity and protease and insect amylase inhibitory activities. However, in gymnosperms, only antifungal activity of defensins has been described thus far. Here, we report antibacterial and insect α-amylase inhibition activities for defensin PsDef1 from P. sylvestris, the first defensin from gymnosperms with a broad range of biological activities described. We also report the solution NMR structure of PsDef1 and its dynamics properties assessed by a combination of experimental NMR and computational techniques. Collectively, our data provide an insight into structure, dynamics, and functional properties of PsDef1 that could be common between defensins from this taxonomic group.