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PDBsum entry 5nce
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Plant protein
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PDB id
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5nce
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PDB id:
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| Name: |
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Plant protein
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Title:
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Structure of psdef1 defensin from pinus sylvestris
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Structure:
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Defensin-1. Chain: a. Fragment: unp residues 34-83. Engineered: yes
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Source:
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Pinus sylvestris. Scots pine. Organism_taxid: 3349. Gene: def1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta-gami plyss b.
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NMR struc:
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15 models
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Authors:
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B.I.Khairutdinov,E.A.Ermakova,E.K.Bessolitsyna,Y.Y.Toporkova, N.B.Tarasova,V.Kovaleva,Y.F.Zuev,I.V.Nesmelova
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Key ref:
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B.I.Khairutdinov
et al.
(2017).
NMR structure, conformational dynamics, and biological activity of PsDef1 defensin from Pinus sylvestris.
Biochim Biophys Acta,
1865,
1085-1094.
PubMed id:
DOI:
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Date:
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03-Mar-17
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Release date:
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07-Jun-17
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PROCHECK
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Headers
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References
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A4L7R7
(DEF1_PINSY) -
Defensin-1 from Pinus sylvestris
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Seq:
Struc:
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83 a.a.
50 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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DOI no:
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Biochim Biophys Acta
1865:1085-1094
(2017)
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PubMed id:
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NMR structure, conformational dynamics, and biological activity of PsDef1 defensin from Pinus sylvestris.
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B.I.Khairutdinov,
E.A.Ermakova,
Y.M.Yusypovych,
E.K.Bessolicina,
N.B.Tarasova,
Y.Y.Toporkova,
V.Kovaleva,
Y.F.Zuev,
I.V.Nesmelova.
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ABSTRACT
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Plants have developed a complex defense response system against pests and
pathogens. Defensins, produced by plants as part of their innate immune
response, form the family of small, basic, cysteine-rich proteins with activity
primarily directed against fungal pathogens. In addition, plant defensins can
show antibacterial activity and protease and insect amylase inhibitory
activities. However, in gymnosperms, only antifungal activity of defensins has
been described thus far. Here, we report antibacterial and insect α-amylase
inhibition activities for defensin PsDef1 from P. sylvestris, the first defensin
from gymnosperms with a broad range of biological activities described. We also
report the solution NMR structure of PsDef1 and its dynamics properties assessed
by a combination of experimental NMR and computational techniques. Collectively,
our data provide an insight into structure, dynamics, and functional properties
of PsDef1 that could be common between defensins from this taxonomic group.
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