PDBsum entry 5m5i

Motor protein

PDB id: 5m5i

Contents

Protein chains

412 a.a.*

426 a.a.*

369 a.a.*

Ligands

GTP

GDP

TA1

ANP

Metals

_MG ×2

* C-alpha coords only

PDB id:

5m5i

Name:

Motor protein

Title:

Pseudo-atomic model of microtubule-bound s.Pombe kinesin-5 motor domain in the amppnp state (based on cryo-electron microscopy experiment): the n-terminus conformation allows formation of a cover neck bundle.

Structure:

Tubulin alpha-1d chain. Chain: a. Tubulin beta-2b chain. Chain: b. Kinesin-like protein cut7. Chain: c. Synonym: cell untimely torn protein 7. Engineered: yes

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Schizosaccharomyces pombe (strain 972 / atcc 24843). Fission yeast. Organism_taxid: 284812. Gene: cut7, spac25g10.07c. Expressed in: escherichia coli bl21(de3).

Authors:

A.Goulet,C.A.Moores,R.A.Cross

Key ref:

M.Britto et al. (2016). Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanism. Proc Natl Acad Sci U S A, 113, E7483. PubMed id: 27834216 DOI: 10.1073/pnas.1611581113

Date:

21-Oct-16 Release date: 30-Nov-16

Protein chain

Q2HJ86  (TBA1D_BOVIN) -  Tubulin alpha-1D chain from Bos taurus

Seq: 452 a.a.

Struc: 412 a.a.*

Protein chain

Q6B856  (TBB2B_BOVIN) -  Tubulin beta-2B chain from Bos taurus

Seq: 445 a.a.

Struc: 426 a.a.*

Protein chain

P24339  (CUT7_SCHPO) -  Kinesin-like protein cut7 from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

Seq: 1085 a.a.

Struc: 369 a.a.

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 3: Chains B, C: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1073/pnas.1611581113

Proc Natl Acad Sci U S A 113:E7483 (2016)

PubMed id: 27834216

Schizosaccharomyces pombe kinesin-5 switches direction using a steric blocking mechanism.

M.Britto, A.Goulet, S.Rizvi, O.von Loeffelholz, C.A.Moores, R.A.Cross.

ABSTRACT

No abstract given.