PDBsum entry 5ltk

Hydrolase

PDB id: 5ltk

Contents

Protein chain

700 a.a.

Ligands

ADP-BEF

SO4 ×2

Metals

_MG

Waters ×4

PDB id:

5ltk

Name:

Hydrolase

Title:

Crystal structure of the prp43-adp-bef3 complex (in hexagonal space group)

Structure:

Pre-mRNA-splicing factor atp-dependent RNA helicase prp43. Chain: a. Engineered: yes

Source:

Chaetomium thermophilum var. Thermophilum dsm 1495. Organism_taxid: 759272. Gene: ctht_0005780. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosetta 2.

Resolution:

3.24Å R-factor: 0.185 R-free: 0.217

Authors:

M.J.Tauchert,R.Ficner

Key ref:

M.J.Tauchert et al. (2017). Structural insights into the mechanism of the deah-Bo helicase prp43.. Elife, 6, . PubMed id: 28092261

Date:

06-Sep-16 Release date: 25-Jan-17

Protein chain

G0RY84  (G0RY84_CHATD) -  RNA helicase from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 764 a.a.

Struc: 700 a.a.

Enzyme reactions

• Enzyme class: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

ATP + H2O = ADP (Bound ligand (Het Group name = ADP) corresponds exactly) + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site