PDBsum entry 5ld2

Hydrolase

PDB id: 5ld2

Contents

Protein chains

1151 a.a.

1121 a.a.

605 a.a.

DNA/RNA

Ligands

ANP

Metals

_MG

PDB id:

5ld2

Name:

Hydrolase

Title:

Cryo-em structure of recbcd+DNA complex revealing activated nuclease domain

Structure:

Recbcd enzyme subunit recb,recbcd enzyme subunit recb, recbcd enzyme subunit recb. Chain: b. Synonym: exodeoxyribonuclease v 135 kda polypeptide, exodeoxyribonuclease v beta chain,exonuclease v subunit recb,exov subunit recb,helicase/nuclease recbcd subunit recb, exodeoxyribonuclease v 135 kda polypeptide,exodeoxyribonuclease v beta chain,exonuclease v subunit recb,exov subunit recb, helicase/nuclease recbcd subunit recb,exodeoxyribonuclease v 135 kda

Source:

Escherichia coli (strain k12). Organism_taxid: 83333. Strain: k12. Gene: recb, ior, rora, b2820, jw2788. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: recc, b2822, jw2790. Gene: recd, hope, b2819, jw2787. Synthetic: yes.

Authors:

M.Wilkinson,Y.Chaban,D.B.Wigley

Key ref:

M.Wilkinson et al. (2016). Mechanism for nuclease regulation in recbcd.. Elife, 5, . PubMed id: 27644322

Date:

23-Jun-16 Release date: 05-Oct-16

Protein chain

P08394  (RECB_ECOLI) -  RecBCD enzyme subunit RecB from Escherichia coli (strain K12)

Seq: 1180 a.a.

Struc: 1151 a.a.*

Protein chain

P07648  (RECC_ECOLI) -  RecBCD enzyme subunit RecC from Escherichia coli (strain K12)

Seq: 1122 a.a.

Struc: 1121 a.a.

Protein chain

P04993  (RECD_ECOLI) -  RecBCD enzyme subunit RecD from Escherichia coli (strain K12)

Seq: 608 a.a.

Struc: 605 a.a.

* PDB and UniProt seqs differ at 21 residue positions (black crosses)

DNA/RNA chain

T-T-T-T-T-T-T-T-T-T-T-T-T-C-T-A-A-T-G-C-G-A-G-C-A-C-T-G-C-T-A-G-C-A-G-T-G-C-T- 51 bases

Enzyme reactions

• Enzyme class 2: Chains B, C: E.C.3.1.11.5 - exodeoxyribonuclease V.
• Reaction: Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.
• Enzyme class 3: Chain B: E.C.5.6.2.4 - Dna 3'-5' helicase.
• Enzyme class 4: Chain D: E.C.5.6.2.3 - Dna 5'-3' helicase.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.