PDBsum entry 5l8w

Structural protein

PDB id: 5l8w

Contents

Protein chains

319 a.a.

512 a.a.

75 a.a.

Ligands

GOL

AYE

Metals

_ZN

Waters ×18

PDB id:

5l8w

Name:

Structural protein

Title:

Structure of usp12-ub-prg/uaf1

Structure:

Ubiquitin carboxyl-terminal hydrolase 12. Chain: a. Synonym: deubiquitinating enzyme 12,ubiquitin thioesterase 12, ubiquitin-hydrolyzing enzyme 1,ubiquitin-specific-processing protease 12. Engineered: yes. Wd repeat-containing protein 48. Chain: b. Synonym: usp1-associated factor 1,wd repeat endosomal protein,p80.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: usp12, ubh1, usp12l1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: wdr48, kiaa1449, uaf1. Gene: ubb. Expressed in: escherichia coli.

Resolution:

2.79Å R-factor: 0.211 R-free: 0.259

Authors:

S.Dharadhar,T.Sixma

Key ref:

S.Dharadhar et al. (2016). A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme. J Struct Biol, 196, 437-447. PubMed id: 27650958 DOI: 10.1016/j.jsb.2016.09.011

Date:

08-Jun-16 Release date: 28-Sep-16

Protein chain

O75317  (UBP12_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase 12 from Homo sapiens

Seq: 370 a.a.

Struc: 319 a.a.

Protein chain

Q8TAF3  (WDR48_HUMAN) -  WD repeat-containing protein 48 from Homo sapiens

Seq: 677 a.a.

Struc: 512 a.a.*

Protein chain

P0CG47  (UBB_HUMAN) -  Polyubiquitin-B from Homo sapiens

Seq: 229 a.a.

Struc: 75 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

DOI no: 10.1016/j.jsb.2016.09.011

J Struct Biol 196:437-447 (2016)

PubMed id: 27650958

A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme.

S.Dharadhar, M.Clerici, W.J.van Dijk, A.Fish, T.K.Sixma.

ABSTRACT

No abstract given.