PDBsum entry 5l8l

Transferase

PDB id: 5l8l

Contents

Protein chains

264 a.a.

109 a.a.

Ligands

ADP

SO4 ×7

EDO ×5

Waters ×348

PDB id:

5l8l

Name:

Transferase

Title:

Aurora-a kinase domain in complex with vnar-d01 (crystal form 1)

Structure:

Aurora kinase a. Chain: a. Synonym: aurora 2,aurora/ipl1-related kinase 1,hark1,breast tumor- amplified kinase,serine/threonine-protein kinase 15,serine/threonine- protein kinase 6,serine/threonine-protein kinase aurora-a. Engineered: yes. Mutation: yes. New antigen receptor variable domain. Chain: b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: aurka, aik, airk1, ark1, aura, ayk1, btak, iak1, stk15, stk6. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: ril. Orectolobus maculatus. Spotted wobbegong.

Resolution:

1.67Å R-factor: 0.193 R-free: 0.223

Authors:

S.G.Burgess,R.Bayliss

Key ref:

S.G.Burgess et al. Allosteric inhibition of aurora-A kinase by a synthet domain. Open biology, . PubMed id: 27411893

Date:

08-Jun-16 Release date: 20-Jul-16

Protein chain

O14965  (AURKA_HUMAN) -  Aurora kinase A from Homo sapiens

Seq: 403 a.a.

Struc: 264 a.a.*

Protein chain

Q8JJ25  (Q8JJ25_9CHON) -  New antigen receptor variable domain (Fragment) from Orectolobus maculatus

Seq: 108 a.a.

Struc: 109 a.a.*

* PDB and UniProt seqs differ at 9 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] (Bound ligand (Het Group name = ADP) corresponds exactly) + ADP + H(+)

L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] (Bound ligand (Het Group name = ADP) corresponds exactly) + ADP + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site