PDBsum entry 5l8h

Hydrolase

PDB id: 5l8h

Contents

Protein chains

311 a.a.

76 a.a.

Metals

_ZN

Waters ×250

PDB id:

5l8h

Name:

Hydrolase

Title:

Structure of usp46-ubvme

Structure:

Ubiquitin carboxyl-terminal hydrolase 46. Chain: a. Synonym: deubiquitinating enzyme 46,ubiquitin thioesterase 46, ubiquitin-specific-processing protease 46. Engineered: yes. Polyubiquitin-b. Chain: b. Engineered: yes. Other_details: recombinant expression followed by chemical synthesis.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: usp46. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ubb. Expression_system_taxid: 562

Resolution:

1.85Å R-factor: 0.168 R-free: 0.194

Authors:

M.Clerici,T.Sixma,S.Dharadhar

Key ref:

S.Dharadhar et al. (2016). A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme. J Struct Biol, 196, 437-447. PubMed id: 27650958 DOI: 10.1016/j.jsb.2016.09.011

Date:

07-Jun-16 Release date: 28-Sep-16

Protein chain

P62068  (UBP46_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase 46 from Homo sapiens

Seq: 366 a.a.

Struc: 311 a.a.*

Protein chain

P0CG47  (UBB_HUMAN) -  Polyubiquitin-B from Homo sapiens

Seq: 229 a.a.

Struc: 76 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

DOI no: 10.1016/j.jsb.2016.09.011

J Struct Biol 196:437-447 (2016)

PubMed id: 27650958

A conserved two-step binding for the UAF1 regulator to the USP12 deubiquitinating enzyme.

S.Dharadhar, M.Clerici, W.J.van Dijk, A.Fish, T.K.Sixma.

ABSTRACT

No abstract given.