PDBsum entry 5l6w

Transferase

PDB id: 5l6w

Contents

Protein chains

290 a.a.

165 a.a.

Ligands

AGS

PDB id:

5l6w

Name:

Transferase

Title:

Structure of the limk1-atpgammas-cfl1 complex

Structure:

Lim domain kinase 1. Chain: l. Synonym: limk-1. Engineered: yes. Cofilin-1. Chain: c. Synonym: 18 kda phosphoprotein,p18,cofilin,non-muscle isoform. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: limk1, limk. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: cfl1, cfl. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.53Å R-factor: 0.229 R-free: 0.284

Authors:

E.Salah,S.Mathea,S.Oerum,J.A.Newman,C.Tallant,R.Adamson,P.Canning, A.Beltrami,F.Von Delft,C.H.Arrowsmith,A.M.Edwards,C.Bountra,S.Knapp, A.N.Bullock

Key ref:

E.Salah and a.n.bullock Structure of the limk1-Atpgammas-Cfl1 complex. To be published, .

Date:

31-May-16 Release date: 08-Jun-16

Protein chain

P53667  (LIMK1_HUMAN) -  LIM domain kinase 1 from Homo sapiens

Seq: 647 a.a.

Struc: 290 a.a.*

Protein chain

P23528  (COF1_HUMAN) -  Cofilin-1 from Homo sapiens

Seq: 166 a.a.

Struc: 165 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chain C: E.C.?
• Enzyme class 3: Chain L: E.C.2.7.11.1 - non-specific serine/threonine protein kinase.
• Reaction:
  1. L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H⁺
  2. L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site